Figure 4

Schematic diagrams of fkbp-lpxC and katA - prlC regions in a subset of meningococcal isolates with class I pilE (A-B) and class II pilE genes (C-D). (A) Open reading frames and pilS cassettes in the region flanking the class I pilE gene were annotated in isolates belonging to cc32. Class I pilE genes were mostly found adjacent to pilS cassettes in the fkbp-lpxC region. (B) The katA-prlC region of cc32 isolates does not contain a class II pilE gene. (C) Open reading frames and pilS cassettes were annotated in the fkbp-lpxC region of isolates belonging to cc5. These strains have three pilS cassettes which are highly conserved. (D) The class II pilE gene is located next to the catalase gene (katA) and is genetically linked to a gene encoding a hypothetical protein (hp). The ID and name of each isolate is indicated. Sequence type and provenance of each strain can be found in Additional files 2 and 3. Diagonal lines represent the end of the contig. X indicates the gene encodes an atypical pilin subunit which is unlikely to result in production of functional Tfp. Putative guanine quartet (G4) and Sma/Cla sequences are shown as black vertical line or hatched boxes, respectively. lpxC: UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase), fkbp: peptidyl-prolyl cis-trans isomerase, chp: conserved hypothetical protein; prlC: putative oligopeptidase A. Scale bars represent 1 kb.