Figure 5

Western blot analysis of SM1 cross reactivity with class I and class II meningococcal pilins. Whole cell extracts from strains with class I or class II pilE genes encoding pilins with different SM1 epitopes, and isogenic mutants lacking pilE were analysed using monoclonal antibody SM1 and anti-peptide antibody EP112606. All strains express pilin, as detected by EP112606. As expected, SM1 reacts with class I pilin in 8013 but does not recognise the class II pilin from FAM18. However, pilin from isolate 92001 which has a class II pilE gene (allele 15) also cross reacts with SM1.