Figure 2

Relative fold change for genes determined by qRT-PCR. The gene expression profile for (A) twenty genes listed in Table 2, (B) the ergosterol pathway genes and (C) in vivo samples of Paracoccidioides recovered directly from systemically infected mouse spleens. Changes in gene expression levels were calculated by relative standard curve method using the control, untreated samples as the calibrator. (D) GST activity was measured in protein extract from Paracoccidioides Pb18 yeast cells grown in the presence or absence of itraconazole. Each error bar represents the standard error of the mean (±SE) from three independent experiments performed in triplicate, and significant fold-changes are denoted by asterisks in the figure (*p ≤ 0.05). Data were normalized to the transcript encoding the α-tubulin protein. Student’s t test was used for statistical comparisons.