Table 2 Oligonucleotide primers used in RDA assays and qRT-PCR
From: Transcriptional profile of Paracoccidioides spp. in response to itraconazole
Sequence name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
Glutathione S-transferase (GST) | GAACCGCAAACCCTAACCCT | ACAGCGGCTGAAAAGTCCCA | 157 |
Chitin synthase regulator 2 (CHSr) | AGAGCTGCAGAATTAGGCCTT | TTTCGCCCGTTCATCTCCGT | 140 |
Betaine aldehyde dehydrogenase (BADH) | GTTGAAGAGCCATTTGGTCC | CAGATCATTGGACCACACAGA | 120 |
Cysteine desulfurase (CYSD) | CAACAGAAGAGATGGAGTATGA | AGCGAATGACACGTTGACACA | 143 |
Ribulose-phosphate 3-epimerase (RP3E) | CAATGGATCGACCTGATATGG | GACCTCCGTCAACTTCGATG | 141 |
Carnitine/acyl-carnitine carrier (CAR) | GAAGGCATTGCCAGGGGGT | CATTATGAACGGGGACGGTG | 139 |
Glutamine amidotransferase subunit pdxT (GLAT) | TGAGAGACTTTGTCAAGAACCA | TGCGCGGATAAATACACCCAT | 143 |
Mfs transporter (MFS) | CTAATTATGTTCTTTTGGGGTAC | GCATCGCCTATACCAACAAGA | 136 |
C6 transcription factor (CTF1B) | CAAACCACTCGTCAACACAATC | GATTGCCTTGAGTCTGATAGAG | 138 |
Acyl-CoA dehydrogenase (ACAD) | GAGAACGAGACGCCCGAAG | GTTGTAGTAAGGACTCTTGTAG | 108 |
GPR1/FUN34/YAAH family protein | ACTGGCTGGGATGTGGGAG | TTCTTCTCCGTCATTTCCTTGA | 141 |
Pyruvate kinase (PK) | ATGCGATGATAAATATCTCTACG | GACACTTGGCGCGGAGAGA | 143 |
Diacylglycerol o-acyltransferase (DGAT) | TATTAGATATACCAAGTGGCCG | TACCCTGGGTTTGTATTCAATG | 143 |
Isovaleryl-CoA dehydrogenase (IVD) | GATGTGGATTACCAACGGGC | TCATGCCAAGCTTGTCGAGTT | 152 |
Ubiquitin-protein ligase (UBI) | GGAGGCATGCAGATCTTCGT | ACGACCGTCCTCAAGCTGC | 168 |
Family integral membrane protein (IMP) | CGCCAGCAATCTGATTATCTC | AACCCAGCTGACCTTCATTAC | 142 |
Heat shock protein (HSP10) | TCTTCCTCCCAGAGAGCGC | CAGGGCTGCCTCCATACTG | 143 |
Heat shock protein (HSP30) | GGCCTTGACAGCATTCTGG | CTGGCGATAAAGGGCAGAAG | 130 |
Heat shock protein (HSP70) | GCAGAAGGAGCTTGAAAGTGT | GTCAACCTCCTCGACAGTAG | 181 |
ATP synthase f0 subunit 9 (ATPS9) | AAGCAGCGAAAATAATGGGATC | GCAAATAATCCTGTAGCTTCTG | 181 |
Lanosterol 14 α-demethylase (ERG11) | CTGAGCTGTAGGGAAAAGTAC | TCCTCAGCGCAAACGTCCTT | 131 |
C5,6-desaturase (ERG3) | GGAGAATATGTATACCAGCCC | ATCCAAGTGATGAGATACAGAG | 128 |
Delta-24-sterol C-methyltransferase (ERG6) | GCTACTCTTACCCGACATTAC | AATGGGCAAGGTAATGTTCATG | 142 |
C-22 sterol desaturase (ERG5) | GGTCCCATGTTCAAAATCCCT | AAATTTGTGGAAAACCGAGACG | 123 |
C-4 methyl sterol oxidase (ERG25) | GGACCATGGCCTACCAAATC | GCGGAGTATTGGTGGTGGAT | 129 |
cDNA* | AGCAGTGGTATCAACGACAGAGTACGCGGG | - | |
CDS* | AAGCAGTGGTATCAACGCAGAGTACT(30)N1N | - | |
PCRII* | AAGCAGTGGTATCAACGCAGAGT | - | |
JBam12* | GATCCGTTCATG | - | |
JBam24* | ACCGACGTCGACTATCCATGAACG | - | |
NBam12* | GATCCTCCCTCG | - | |
NBam24* | AGGCAACTGTGCTATCCGAGGGAG | - | |
RBam12* | GATCCTCGGTGA | - | |
RBam24* | AGCACTCTCCAGCCTCTCACCGAG | - | |
T7* | GTAATACGACTCACTATAGGGC | - | |
Oligo (dT)15* | AAGCAGTGGTATCAACGCAGAGTACT(30)N1N | - |