Figure 10

NAHR-based deletions. a) Illustration of NAHR-based deletions. A region of the Hawaiian genome is sequenced generating reads 1 to 5. These reads span a segment that we call ‘S’, that corresponds to the anchoring segment that generated a previous NAHR event. Once reads 1 to 5 are sequenced in the Hawaiian strain, the alignment of these to the N2 reference strain are such that those within the ‘S’ segment map non-uniquely (reads 2, 3 and 4), whereas those encompassing regions outside of ‘S’ should map uniquely to the genome (reads 1 and 5). The deleted region in the Hawaiian genome is expected to have no coverage in the N2 reference genome. b) A deletion generated by a NAHR event. This deletion is reported as niDf57 (II) by WormBase, based on the study of [48]. Reads in green indicate those aligned on the positive strand, whereas reads in yellow indicate those aligned on the negative strand.