Figure 1
Media copper concentrations influence respiratory, but not fermentative, growth in yeast. (A) Dose–response matrix measuring the combinatorial effects of copper sulfate (CuSO4) and bathocuproine disulphonic acid (BCS, a copper chelator) on respiratory growth. Yeast (BY4743) was grown under respiring conditions (YPE media) in increasing concentrations of CuSO4 (see right) and BCS (see top). Optical density (i.e. OD600, y-axis) of each culture is plotted over time (x-axis). Each condition is colored based on growth relative to the untreated condition (i.e. no BCS and no CuSO4 added; marked with an asterisk and represented by the grey curve). Green and red indicate increased and decreased growth relative to this reference, respectively (see legend below). Growth data highlighted in yellow and blue were used in (B) and (C), respectively. (B) Dose–response curves illustrating the effect of CuSO4 (x-axis) on yeast growth. Growth relative to that in the absence of CuSO4 is plotted on the y-axis, and was measured in both fermenting (YPD) and respiring (YPE) conditions (indicated in black and red, respectively). The mean of three replicates is plotted; error bars represent the standard deviation. (C) Similar to (B) but measuring the effects of BCS (x-axis).