Figure 4

Pharmacological rescue of Cu-deficiency phenotypes. (A) Chemical structure of disulfiram (DSF) and elesclomol (ES). (B) Dose–response curves measuring the sensitivity of respiring yeast (BY4743 grown in YPE) to increasing concentrations of BCS (indicated on x-axis), in the presence of 0.228 μM DSF (black) or 0.228 μM ES (green). Growth relative to that in the absence of BCS is indicated by the y-axis. The mean of three replicates are plotted; error bars indicate the standard deviation. (C) Respiratory growth of the ctr1Δ/ctr1Δ strain in response to various concentrations of DSF (indicated on the x-axis). Growth after 40 hours in YPE was measured by area under the curve (y-axis). The mean of 3 replicates is plotted. Error bars indicate the standard deviation. Representative growth curves are shown on the right. (D) Similar to (C) only involving ES.