Himar1 transposon insertion site in the A. marginale str. Virginia genome. ACT (Artemis Comparison Tool) window showing alignment between the A. marginale genome (CP000030) used as reference, the A. marginale str. Virginia omp10::himar1 contig formed by Roche/454 and Illumina sequencing reads and the Himar1 transposon sequences. Alignment between the A. marginale str St Maries and the omp10::himar1 mutant shows that sequences flanking the transposon insertion site are highly similar sharing an identity of 99% (matching red band). This demonstrates that the transposon insertion site (yellow boxes) occurred at nucleotide 245 after the first base of the omp10 start codon (black dotted line) in the reference strain. Alignment with the Himar1 transposon sequences clearly show the insertion of these sequences in the omp10::himar1 mutant which are not present in the A. marginale str. St Maries (absence of matching band).