Figure 4
From: Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis
Characterization of Himar1 transposon insertion site. A. Artemis (genome browser and annotation tool) window showing the A. marginale genome (CP000030) used as a reference for the location of the omp10 gene (AM1223, 1092273–1093555), and the TA dinucleotide (1093290–1093291) at the Himar1 tn insertion site (arrow) determined by high throughput genome sequencing analysis. B. Himar1 tn insertion into the omp10 gene was mediated by the A7 transposase in a cut and paste mechanism leading to the duplication of TA dinucleotide sequences. A. marginale genome (underlined uppercase text, TA dinucleotide duplications (enhanced uppercase text) flanking the tn elements (bold lowercase).