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Figure 7 | BMC Genomics

Figure 7

From: Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis

Figure 7

Relative gene expression by RT-qPCR. A. location of binding sites for primers and probes designed to target omp8 (1. AB1591, 2. AB1592, 3. AB1593), omp9 (4. AB1581, 5. AB1582, 6. AB1583), 3’ end of omp10 (7. AB1569, 8. AB1570, 9. AB1571), and the 5’ end of omp10 (10. AB1594, 11. AB1595, 12. AB1596). B. Bar lengths represent the percentage of expression of omp8, omp9, 3’ end of omp10 and 5’ end of omp10 in A. marginale wild-type (red bars) and omp10::himar1 mutant (blue bars). msp5, rpoH and 16S rRNA were used as reference genes for data normalization. Changes in expression of these genes were calculated using the 2-ΔΔCtmethod.* Significant differences (P < 0.05) were calculated as described in materials and methods.

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