Figure 8

Immunoblotting of omp10::himar1 mutant and WT A. marginale using the specific monoclonal antibody Omp9. Proteins from equal amounts of host cell-free wild-type (WT) and omp10::himar1 A. marginale were separated by SDS-PAGE gel electrophoresis. Immunoblot PVDF membranes of transferred proteins were reacted with monoclonal antibodies and reactions were visualized by chemiluminescence. A. Monoclonal antibody Omp9 (4 μg/ml) with specificity to Omp9 protein (40 kDa) (black arrow). B. Negative control, monoclonal Tryp1E1 (4 μg/ml) (exhibits specificity for a variable surface glycoprotein of Trypanosoma brucei. C. Monoclonal F16C1 (2 μg/ml), reacts with the Msp5 (19 kDa) (blue arrow) protein of A. marginale, was used as loading control. A. marginale str. Virginia and uninfected ISE6 cells were used as positive and negative controls respectively.