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Table 1 PCR and Taqman qPCR oligonucleotides used in this study

From: Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis

Oligonucleotide sequence (5’ to 3’)

Target

Size

Reference

PCR

    

AB1553

CTCCAATCGGAGGGGTTGTG

omp6

492bp

[43]

AB1554

GCATAAATCCAGTTTAGCCTCC

AB1555

GTGGTTAGATCTTTTCTGTTGGG

omp7

399bp

[43]

AB1556

CGCTCTACCACTGACCTTCATG

AB1591

GCTGGAGTTCGAAGCGATGC

omp8

259bp

This study

AB1592

CAGAGCGCCCTGTTTCAGTG

AB1559

AGCTGGGGCTCTTGCGTTTG

omp9

1096bp

[43]

AB1560

AACATATTCACTATAATCTGACGCTGC

AB1561

TCCTTCGGGTTGCTGCGTTG

omp10

969bp

[43]

AB1562

GCTTACCCCCATTCCAGCAC

AB1572

AGGATGATCAGCCACACTGGAA

16S

131bp

This study

AB1573

TACAACCCTAAGGCCTTCCTCA

**qPCR

AB1591

GCTGGAGTTCGAAGCGATGC

AB1592

CAGAGCGCCCTGTTTCAGTG

omp8

259bp

This study

AB1593

GCGTGAGCACTGCGGTACAGACGG

AB1581

GAAGTCACTACACGACCTGACTGT

AB1582

TAAAGCATCTTCGCGGGTCGT

omp9

145bp

[43]

AB1583

TATTCAGTGCGCTGAACACTGCGATCCA

AB1594

GTGGGTGCTGTACGCACATT

AB1595

AAAGACAGCAGGCAGCAACA

omp10-5'

170bp

This study

AB1596

CGCGTGTCCTTCGGGTTGCT

AB1569

GGTGCTGAGTTGAAGCTTGC

AB1570

GCCACAGACCCACTATCAGC

omp10-3'

140bp

[43]

AB1571

TATCTCGCGCTGCATCGGTG

AB1572

AGGATGATCAGCCACACTGGAA

AB1573

TACAACCCTAAGGCCTTCCTCA

16S

131bp

[42]

AB1574

TATTGGACAATGGGCGCAAGCCTGAT

AB1606

CTCACAGGCGAAGAAGCAGAC

AB1607

GCCCGACATACCTGCCTTT

msp5

145bp

[55]

AB1610

TGGGCGACAAGAAGCCAAGTGA

AB1608

ATCAAAGCTATTGCGGAGGA

AB1607

ACAGAACTCTCCCCATGCAC

rpoH

116bp

This study

AB1611

TGCCAATCGGGACGTTTCGC

AB1242

AAAACAGGCTTACCGCTCCAA

AB1243

GGCGTGTAGCTAGGCTCAAAGT

opag2

151bp

[41]

AB1250

CTCTCCTCTGCTCAGGGCTCTGCG

  1. *Primers and TaqMan probes used were manufactured at Eurofins MGM Operon.
  2. Oligonucleotides are labeled with 6-Carboxyfluorescein 6-FAM at the 5’ end and Tetramethylrhodamine TAMRA at the 3’ end.