Table 1 French specific canSNPs and primer sequences used for HRM analysis
canSNP | Position* | Target | SNP | Forward primer (5’-3’) | Reverse primer (5’-3’) | Product size (bp) |
|---|---|---|---|---|---|---|
BA15 | 126639 | Alps (B.CNEVA) | A to G | CCACAAGGTGGAATTATTACTAAAGA | GGTTCACCTGTTTTCGGATCT | 80 |
BA16 | 3765357 | Pyrenees (B.CNEVA) | A to G | GGTGGTTTCGGATATGCACT | AAAGGTGCTGGGGTAGTAAGG | 68 |
BA17 | 4719494 | MC + SL (B.CNEVA) | G to A | TTAGATCTCGTTTTCGGTTCC | CAATGAGTGTACGGCTCCAA | 79 |
BA18 | 2390832 | SL (B.CNEVA) | T to C | CCAGGCAAATACATTGTGGA | TTACAGTCTGTGTTGCCGTTG | 73 |
BA19 | 2573536 | French B.CNEVA | A to G | CATATATTTTCACCTCTTTTATGAACA | GATAAAAGGCTGTCGGATGG | 90 |
BA20 | 3434997 | Doubs (A.001/002) | A to C | AGCGAGCCAATTTTGGAACCGA | AGGCGGGATTGTTGGTGGATGT | 63 |
BA21 | 3562427 | Central NE (A.011/009) | A to C | AGCAAAAAGTCGGCAAAGAA | ACAGAGCTTCCTCCGAACTG | 82 |
BA22 | 820195 | NE (A.011/009) | T to C | AGTGGTGCAATCCCAATTTC | CGCAGCAATATTCGCTATCA | 99 |