Figure 7
From: BisQC: an operational pipeline for multiplexed bisulfite sequencing
Frequency of methylation per CpG and mean CpG coverage as QC metrics. A, C, E, G) The frequency of methylation at any CpG region has very similar patterns across samples (A, C, E, G). In adult human brain tissue, >50% of all CpGs show 0% methylation and >20% of CpGs are fully methylated. A minority of CpGs lies within the extremes. B, D, F, H) Histograms denote average coverage per CpG in bins and where the X-axis is on the log10 scale (1 = 10X coverage). The purpose of performing these analyses is to assess if any sample deviates substantially from other samples. If so, these samples should be carefully re-investigated or re-processed. Here, the histogram pattern across samples B, D, F, and H are very similar.