Table 1 Sequences and specific modifications of oligonucleotides used in the BisQC protocol
From: BisQC: an operational pipeline for multiplexed bisulfite sequencing
Name | Sequence (5′ to 3′) |
|---|---|
Methylated C adaptor: mC-PE1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCsT-OH |
Methylated C adaptor: mC-PE2 | p-GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-OH |
PCR primer: IndPEPCR_F | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCsT |
PCR primer: IndPEPCR_R | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCsT |
PE-qPCR_F (Phix-PE-qPCR_F) | AATGATACGGCGACCACCGA-OH |
PE-qPCR_R (Phix-PE-qPCR_R) | CAAGCAGAAGACGGCATACGA-OH |
Index_1R primer | CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTC-OH |
Index_2R primer | CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTC-OH |
Index_3R primer | CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTC-OH |
Index_4R primer | CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTC-OH |
Index_5R primer | CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTC-OH |
Index_6R primer | CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTC-OH |
Index_7R primer | CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTC-OH |
Index_8R primer | CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTC-OH |
Index_9R primer | CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTC-OH |
Index_10Rprimer | CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTC-OH |
Index_11Rprimer | CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTC-OH |
Index_12Rprimer | CAAGCAGAAGACGGCATACGAGATGAACATGTGACTGGAGTTC-OH |