Figure 1
From: Altering gene expression by aminocoumarins: the role of DNA supercoiling in Staphylococcus aureus
Changes in gene expression by aminocoumarins is due to gyrase inhibition. Strain HG001 and HG001, nov142 were grown to exponential phase (OD600 = 0.6) and treated with novobiocin (novo, 0.5 mg/L), simocyclinone D8 (SD8, 4 mg/L), clorobiocin (cloro, 0.125 mg/L), and ciprofloxacin (cipro, 40 mg/L) for 10 min. RNA was hybridized with a digoxigenin-labelled recA as well as gyrB and arlR specific probes.