Figure 4

Validation of the microarray results by qPCR analysis. CCL8 (chemokine (C-C motif) ligand 8) and IRF7 (interferon regulatory factor 7) expression values (normalized based on Ćāactin and Ubiquitin B expression values) in oviductal samples inseminated with X-spermatozoa compared to oviductal samples inseminated with Y-spermatozoa. The expression of both transcripts in the oviductal samples inseminated with Y-spermatozoa was significantly different from that of the oviductal samples inseminated with Y-spermatozoa (Pā<ā0.05).