Figure 1

Pulsed field gel electrophoresis (PFGE) analysis of the chromosome pattern of R. miehei CAU432. Dark bands indicate the chromosome markers of Saccharomyces cerevisiae and S. pombe. White bands indicate the group bands of R. miehei chromosomes. The gel was electrophoresed under three different conditions: (a) Full set chromosome electrophoresis for 96 h with a switch interval ramp from 20 min to 40 min. (b) The middle range chromosome separation, gel electrophoresis for 60 h with a switch interval ramp from 20 min to 30 min, followed by 36 h with a switch interval ramp from 30 min to 40 min. (c) Large chromosomes electrophoresis for 96 h with a switch interval ramp from 40 min to 50 min. (d) The summary of estimated chromosome band molecular sizes on the PFGE gel.