Figure 2

Multiple sequence alignment of α-KTx precursors and amino acid sequences from Mesobuthus gibbosus. A) Mgib23 deduced amino acid sequence and related toxins. B) Mgib29 precursor and all members of the subfamily α-KTx14.x. C) Mgib2 precursor sequence and the sole characterized member of the α-KTx26.x subfamily. D) MgibC5 and related precursor sequences. Signal peptides are shown in lowercase; sequences in bold and capital letters correspond to mature sequences or described toxin sequences; identical residues of mature sequences are highlighted in different colours according to the region or putative group (in more than 50% of the corresponding subfamily sequences). Number of residues, identity (% I) and E-values are shown on the right. Identity and E-values correspond to the mature sequence regions. Precursor organization is shown by cartoon of the gene (top of the figure): signal peptide is shown in grey line; pro-peptide and mature peptide is shown in green.