Figure 3
Multiple sequence alignment of β-KTx precursors and peptides and deduced sequences from Mesobuthus gibbosus. β-KTxs amino acid sequences were aligned with ClustalX. Signal peptide are shown in grey and lower case; sequence in bold and capital letters correspond to mature sequences or the N-terminal region of the described toxins; identical residues of mature sequences are highlighted in different colour according to the region or putative group; residues in yellow show identical residues in all the β-KTx class I; Number of residues, identity (% I) and E-values are shown on the right. Identity and E-values correspond to the mature sequences. Abbreviations of scorpion names, accession number of the database and toxin names are show to the left. Abbreviations of scorpion scientific names correspond to: Anau, Androctonus australis; Buoc, Buthus occitanus Israelis; Meup, Mesobuthus eupeus; Mgib, Mesobuthus gibbosus; Mema, Mesobuthus martensii; Tcos, Tityus costatus; Tser, Tityus serrulatus; Tsti, Tityus stigmurus; Ttri, Tityus trivitatus; Tdis, Tityus discrepans; Lymu, Lychas mucronatus. Precursor organization is shown by cartoon of the gene (top of the figure): signal peptide is shown in grey line; pro-peptide and mature peptide is shown in green; intron region is symbolized by triangles in front of the toxin TtrbetaKTx as unique example of the genomic organization of the class I [14].