Figure 7

Multiple sequence alignment of Meg toxins and related precursors of the chlorotoxin group. Amino acid number and targets are shown on the right of the multiple alignments. Precursor denotes the deduced sequence from gene or transcript. Mature sequences are shown in bold (according to the references of the signal peptide prediction or pure peptide). Filled circle corresponds to amidated C-terminus (Arginine amide). Cysteines residues are highlighted in red. Residues highlighted in pink correspond to ≥90% identity and green to ≥50% identity. Symbol ¹, corresponds to the deduced sequence based on amino acid analysis reported by Tytgat et al. 1998 [52]. Symbol ², corresponds to the deduced sequence based on genome analysis reported by Cao et al. 2013 [46]. Symbol ³, corresponds to the activity reported by Rosso and Rochat 1985 [55]. Symbol ⁴, means activity reported only in UniProtKB database (data not confirmed by reference of the authors). ClCh, Chloride channel ligand; InGlioma cells, inhibition and invasion of glioma cells expressing CLCN/ClC-3 voltage-gated chloride channels. Abbreviations of scorpion scientific names correspond to: Aau, Androctonus australis; Ama, Androctonus mauritanicus mauritanicus; Boc, Buthus occitanus Israelis; Hju, Hottentotta judaicos; Lqh, Leiurus quinquestriatus hebraeus; Lqu, Leiurus quinquestriatus quinquestriatus; Meu, Mesobuthus eupeus; Mgi, Mesobuthus gibbosus; Mta, Mesobuthus tamulus; Psc, Parabuthus schlechteri.