Figure 3

Expression profiles of MKL1/2 and ELK4 dependent target genes in response to zymosan treatment. (A) and (B) Expressions of MKL1/2 dependent genes (Cfl1, Dstn, Vcl and Thbs1) and ELK4 dependent genes (Il10, c-fos, Egr1 and Egr3) were analyzed by Q-PCR. Thioglycollate-elicited macrophages were transfected with NS or specific siRNAs for MKL1/2 or ELK4, as indicated, and treated with or without zymosan for 1 h. (C) Srf mRNA expression in response to MKL1/2 or ELK4 knockdown under basal conditions or after treatment with zymosan for 1 h. Target gene expression is represented as the average fold change compared to NS siRNA transfected samples of three independent experiments. **p < 0.01 vs. NS; *p < 0.05 vs. NS. Error bars represent standard deviations.