Figure 7

Validation of MKL1 and ELK4 recruitment to target gene promoters following zymosan treatment. (A) MKL1 ChIP assay was performed in BLRP-MKL1-BirA stable cells under basal conditions and after zymosan treatment for 1 h. Immunoprecipitated DNA was analyzed by Q-PCR using primers flanking the proximal promoter regions of Srf and Vcl. (B) Conventional ELK4 ChIP assay was performed in BLRP-MKL1-BirA stable cells under basal conditions and after zymosan treatment for 1 h. Rabbit IgG was used as a negative control. Immunoprecipitated DNA was analyzed by qPCR using primers flanking the proximal promoter regions of c-fos and Egr1.