Transcriptome assembly and microarray construction for Enchytraeus crypticus, a model oligochaete to assess stress response mechanisms derived from soil conditions

Table 1 Sequencing results and assembly statistics (using Newbler) for E. crypticus transcriptome sequences

Sequencing results	Total	Aligned	Assembled
Number of reads¹	1,478,792	1,351,016	1,273,500
Number of bases	619,758,522	569,108,879	663,968,932
Assembly statistics	Singletons	Contigs	Isotigs
Number of sequences	87,686	27,296	24,748
Number of bases	34,418,269	28,992,517	31,886,676
Average sequence size (bp)	392.	1,062	1,288
Length of N50 sequence (bp)²	475	1,354	1,443
Largest sequence size (bp)	1,195	6,693	7,689

¹After quality filtering steps and removal of outliers such as adaptor sequences and repeats.
²N50 is a weighted median statistic, such that 50% of all bases are contained in sequences not inferior to N50 length.