Figure 1

The Tobacco Mitochondrial Transcriptome. A – Illustration of transcript depth of coverage for the Nicotiana tabacum mitogenome. The figure was generated using abundance data from Lasergene’s SeqMan Pro v. 3 (DNASTAR, Madison, WI, USA) which were converted to circular coordinates using a custom perl script and drawn by gnuplot (http://www.gnuplot.info). The inner circle shows the location of the genes and was generated from GenBank accession NC_006581 using Organellar Genome DRAW[33]. B – Higher-resolution view of the first 35,000 bp of the mitogenome. The depth-of-coverage chart was generated using Lasergene’s Seqman Pro v. 3. Protein-coding genes (black boxes) and open reading frames (ORFs, yellow boxes) were manually placed below each area based on a finer nucleotide map. Arrows represent the predicted transcription direction for each transcribed area.