Figure 2

qRT-PCR Analysis of Tobacco Mitogenome ORF Transcript Abundance. Total RNA was extracted from three biological replicates of leaf (A), root (B), and flower (C) tissues and quantified using reverse transcriptase quantitative PCR. Transcript abundance was calculated using a derivation of the methodology of Alvarez et al.[34]. All values were normalized to cox2 abundance as a control. DNA contamination was estimated by RT minus controls and calculated values were subtracted from the measured transcript abundance.