Positive and negative interactions in the oral biofilm active microbiota. A – Relative abundance of bacterial genera (based on 16S assignment, see Methods) and fluctuation through time were studied in a pairwise manner calculating the Spearman’s rho parameter (see Methods). Genera labels are highlighted in color according to phylum: Actinobacteria (pink), Bacteroidetes (Orange), Fusobacteria (Green), Firmicutes (Blue), Proteobacteria (Black), and Spirochaetes (Grey). B – Detail of the Spearman correlations found in the analysis. The left panel shows a strong negative (ρ ~ - 0.75) correlation between Actinomyces and Aggregatibacter through time. In the right panel, a positive correlations is presented among late colonizers such as Leptotrichia, Fusobacterium, Porphyromonas, and Bacteroides (ρ ~ 0.80).