Figure 1
Detection of autoinducers butanoyl-homoserine lactone (C 4 -HSL) and 3-oxo-dodecanoyl homoserine lactone (3O-C 12 -HSL) by thin layer chromatography (TLC) when different P. aeruginosa strains were grown at 30°C and 37°C. The first lane of each strain corresponds to cultures grown at 30°C and the second to cultures grown at 37°C. AHLs extraction was performed as described in Experimental procedures. In (A) the plate was overlaid after chromatography with the short-chain AHL biosensor C. violaceum CV026 and in (B) the silica TLC plate was run in methanol 60% for 4 h and overlaid after chromatography with the long-chain AHL biosensor E. coli (pSB1075).