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Table 2 Compilation of polypeptides identified both in the silver stained gels and in the autoradiogram

From: The link between transcript regulation and de novo protein synthesis in the retrograde high light acclimation response of Arabidopsis thaliana

  Protein name ATG number MM (kDa) Localization Functional role Silver De novo Mascot score
1. 2-Cys Peroxiredoxin AT3G11630 22.4 Chloroplast Defense response + + 66
2. 3-Ketoacyl CoA thiolase 3 AT2G33150 48.6 Peroxisome Fatty acid biosynthesis + + 99
3 Ascorbate peroxidase 1 AT1G07890 27.6 Cytosol Defense response + + 253
4. ATP synthase delta-subunit AT4G09650 22.8 Chloroplast ATP synthesis + + 260
5. ATP synthase subunit beta ATCG00480 47.7 Chloroplast ATP synthesis + + 365
6. Carbonic anhydrase 1 AT3G01500 25.6 Chloroplast Carbon utilization + + 45
7. Carbonic anhydrase 2 AT5G14740 25.1 Chloroplast Carbon utilization + + 72
8. Chaperonin 60 beta AT1G55490 58.1 Chloroplast Protein folding + + 89
9. Chloroplast HSP 70-1 AT4G24280 74.6 Chloroplast Stress response + + 528
10. Chloroplast HSP 70-2 AT5G49910 74.6 Chloroplast Stress response + + 337
11. Cu/Zn Superoxide dismutase AT2G28190 15.7 Chloroplast Defense response + + 81
12. Cyclophilin Cyp 20-3 AT3G62030 19.7 Chloroplast Rotamase + + 207
13. Dehydroascorbate reductase AT1G19570 21.7 Cytosolic Defense response + + 93
14. D-Ribulose-5-P epimerase AT5G61410 28 Chloroplast Calvin cycle + ND 154
15. Fructose-bisphosphate aldolase 1 AT2G21330 41.9 Chloroplast Calvin cycle + + 91
16. Fructose-bisphosphate aldolase 2 AT4G38970 38 Chloroplast Calvin cycle + + 106
17. GAP C2 subunit AT1G13440 36.9 Cytosol Oxidoreductase + + 164
18. Germin 3 oxalate oxidase AT5G20630 19.5 Apoplast Defense response + ND 243
19. Glutamine synthetase 2 AT5G35630 42.5 Chloro/Mito Glutamine biosynthesis + + 141
20. Glutathione S-transferase F8 AT2G47730 23.9 Chloroplast Stress response + ND 71
21. Glutathione S-transferase F9 AT2G30860 24.2 Cytosol Stress response + ND 118
22. Glyceraldehyde-3-P-DH, B subunit AT1G42970 39.3 Chloroplast Calvin cycle + + 70
23. HCF 136 AT5G23120 38.5 Chloroplast Photosynthesis + + 173
24. Lactate/malate dehydrogenase AT1G53240 33.2 Mitochond. TCA-cycle + + 65
25. Malate dehydrogenase AT3G47520 34 Chloroplast Redox metabolism + + 107
26. Manganese SOD AT3G10920 22.2 Mitochond Defence response + ND 78
27. O-Acetyl serine thiol lyase B AT2G43750 35.1 Chloroplast Cysteine biosynthesis + + 85
28. Phosphoglycerate kinase 1 AT1G79550 42.63 Chloroplast Calvin cycle + + 86
29. Phosphoglycerate mutase AT3G08590 60.7 Cytosol Glycolysis -+ + 143
30. Phosphoribulokinase AT1G32060 39.2 Chloroplast Calvin cycle + + 97
31. Plastid-lipid-associated protein 1 AT4G04020 34.9 Chloroplast Stress response + + 113
32. Plastocyanin (DRT 112) AT1G20340 10.5 Chloroplast Electron transport + ND 169
33. PSII oxygen evolving complex AT5G66570 26.5 Chloroplast Photosynthesis + + 114
34. PSII, subunit PSB-O2 AT3G50820 35.0 Chloroplast Photosynthesis + + 304
35. PSII subunit P-1 AT1G06680 20.2 Chloroplast Photosynthesis + + 183
36. Ribose 5-phosphate isomerase AT3G04790 27.1 Chloroplast Calvin cycle + ND 161
37. Ribosomal protein S1 AT5G30510 40.5 Chloroplast RNA binding + + 70
38. RPL12 | ribosomal protein L12-A AT3G27830 14 Chloroplast Translation + + 78
39. Rubisco activase AT2G39730 46.2 Chloroplast Calvin cycle + + 462
40. RubisCO large subunit ATCG00490 53 Chloroplast Calvin cycle + + 304
41. RubisCO small subunit 1A AT1G67090 14.7 Chloroplast Calvin cycle + + 346
42. RubisCO small subunit 1B AT5G38430 14.8 Chloroplast Calvin cycle + + 71
43. RubisCO small subunit 2B AT5G38420 14.8 Chloroplast Calvin cycle + + 308
44. S-Adenosylmethionine synthetase 1 AT1G02500 43.2 Cytoplasm Met adenos.transferase + ND 92
45. Sedoheptulose-bisphosphatase AT3G55800 36.1 Chloroplast Calvin cycle + + 229
46. Stromal APx AT4G08390 37.8 Chloroplast Defense response + + 67
47. Thioredoxin m1 AT1G03680 12.4 Chloroplast Defense response + + 105
48. Thioredoxin m2 AT4G03520 12.5 Chloroplast Defense response + + 72
49. Triose phosphate isomerase AT2G21170 27 Chloroplast Calvin cycle + + 133
  1. Shown are the specific details about size, predicted localization, the functional role of the proteins and the MASCOT score. MG #: number in annotated master gel; +: Unequivocally identified by mass spectrometry with at least two peptides; +-: tentatively identified by one peptide; ND: not detected. Polypeptides #5 and 40 are plastome-encoded.
  2. ND- not detected in autoradiograms.