The link between transcript regulation and de novo protein synthesis in the retrograde high light acclimation response of Arabidopsis thaliana

Oelze, Marie-Luise; Muthuramalingam, Meenakumari; Vogel, Marc Oliver; Dietz, Karl-Josef

doi:10.1186/1471-2164-15-320

Table 2 Compilation of polypeptides identified both in the silver stained gels and in the autoradiogram

From: The link between transcript regulation and de novo protein synthesis in the retrograde high light acclimation response of Arabidopsis thaliana

	Protein name	ATG number	MM (kDa)	Localization	Functional role	Silver	De novo	Mascot score
1.	2-Cys Peroxiredoxin	AT3G11630	22.4	Chloroplast	Defense response	+	+	66
2.	3-Ketoacyl CoA thiolase 3	AT2G33150	48.6	Peroxisome	Fatty acid biosynthesis	+	+	99
3	Ascorbate peroxidase 1	AT1G07890	27.6	Cytosol	Defense response	+	+	253
4.	ATP synthase delta-subunit	AT4G09650	22.8	Chloroplast	ATP synthesis	+	+	260
5.	ATP synthase subunit beta	ATCG00480	47.7	Chloroplast	ATP synthesis	+	+	365
6.	Carbonic anhydrase 1	AT3G01500	25.6	Chloroplast	Carbon utilization	+	+	45
7.	Carbonic anhydrase 2	AT5G14740	25.1	Chloroplast	Carbon utilization	+	+	72
8.	Chaperonin 60 beta	AT1G55490	58.1	Chloroplast	Protein folding	+	+	89
9.	Chloroplast HSP 70-1	AT4G24280	74.6	Chloroplast	Stress response	+	+	528
10.	Chloroplast HSP 70-2	AT5G49910	74.6	Chloroplast	Stress response	+	+	337
11.	Cu/Zn Superoxide dismutase	AT2G28190	15.7	Chloroplast	Defense response	+	+	81
12.	Cyclophilin Cyp 20-3	AT3G62030	19.7	Chloroplast	Rotamase	+	+	207
13.	Dehydroascorbate reductase	AT1G19570	21.7	Cytosolic	Defense response	+	+	93
14.	D-Ribulose-5-P epimerase	AT5G61410	28	Chloroplast	Calvin cycle	+	ND	154
15.	Fructose-bisphosphate aldolase 1	AT2G21330	41.9	Chloroplast	Calvin cycle	+	+	91
16.	Fructose-bisphosphate aldolase 2	AT4G38970	38	Chloroplast	Calvin cycle	+	+	106
17.	GAP C2 subunit	AT1G13440	36.9	Cytosol	Oxidoreductase	+	+	164
18.	Germin 3 oxalate oxidase	AT5G20630	19.5	Apoplast	Defense response	+	ND	243
19.	Glutamine synthetase 2	AT5G35630	42.5	Chloro/Mito	Glutamine biosynthesis	+	+	141
20.	Glutathione S-transferase F8	AT2G47730	23.9	Chloroplast	Stress response	+	ND	71
21.	Glutathione S-transferase F9	AT2G30860	24.2	Cytosol	Stress response	+	ND	118
22.	Glyceraldehyde-3-P-DH, B subunit	AT1G42970	39.3	Chloroplast	Calvin cycle	+	+	70
23.	HCF 136	AT5G23120	38.5	Chloroplast	Photosynthesis	+	+	173
24.	Lactate/malate dehydrogenase	AT1G53240	33.2	Mitochond.	TCA-cycle	+	+	65
25.	Malate dehydrogenase	AT3G47520	34	Chloroplast	Redox metabolism	+	+	107
26.	Manganese SOD	AT3G10920	22.2	Mitochond	Defence response	+	ND	78
27.	O-Acetyl serine thiol lyase B	AT2G43750	35.1	Chloroplast	Cysteine biosynthesis	+	+	85
28.	Phosphoglycerate kinase 1	AT1G79550	42.63	Chloroplast	Calvin cycle	+	+	86
29.	Phosphoglycerate mutase	AT3G08590	60.7	Cytosol	Glycolysis	-+	+	143
30.	Phosphoribulokinase	AT1G32060	39.2	Chloroplast	Calvin cycle	+	+	97
31.	Plastid-lipid-associated protein 1	AT4G04020	34.9	Chloroplast	Stress response	+	+	113
32.	Plastocyanin (DRT 112)	AT1G20340	10.5	Chloroplast	Electron transport	+	ND	169
33.	PSII oxygen evolving complex	AT5G66570	26.5	Chloroplast	Photosynthesis	+	+	114
34.	PSII, subunit PSB-O2	AT3G50820	35.0	Chloroplast	Photosynthesis	+	+	304
35.	PSII subunit P-1	AT1G06680	20.2	Chloroplast	Photosynthesis	+	+	183
36.	Ribose 5-phosphate isomerase	AT3G04790	27.1	Chloroplast	Calvin cycle	+	ND	161
37.	Ribosomal protein S1	AT5G30510	40.5	Chloroplast	RNA binding	+	+	70
38.	RPL12 \| ribosomal protein L12-A	AT3G27830	14	Chloroplast	Translation	+	+	78
39.	Rubisco activase	AT2G39730	46.2	Chloroplast	Calvin cycle	+	+	462
40.	RubisCO large subunit	ATCG00490	53	Chloroplast	Calvin cycle	+	+	304
41.	RubisCO small subunit 1A	AT1G67090	14.7	Chloroplast	Calvin cycle	+	+	346
42.	RubisCO small subunit 1B	AT5G38430	14.8	Chloroplast	Calvin cycle	+	+	71
43.	RubisCO small subunit 2B	AT5G38420	14.8	Chloroplast	Calvin cycle	+	+	308
44.	S-Adenosylmethionine synthetase 1	AT1G02500	43.2	Cytoplasm	Met adenos.transferase	+	ND	92
45.	Sedoheptulose-bisphosphatase	AT3G55800	36.1	Chloroplast	Calvin cycle	+	+	229
46.	Stromal APx	AT4G08390	37.8	Chloroplast	Defense response	+	+	67
47.	Thioredoxin m1	AT1G03680	12.4	Chloroplast	Defense response	+	+	105
48.	Thioredoxin m2	AT4G03520	12.5	Chloroplast	Defense response	+	+	72
49.	Triose phosphate isomerase	AT2G21170	27	Chloroplast	Calvin cycle	+	+	133

Shown are the specific details about size, predicted localization, the functional role of the proteins and the MASCOT score. MG #: number in annotated master gel; +: Unequivocally identified by mass spectrometry with at least two peptides; +-: tentatively identified by one peptide; ND: not detected. Polypeptides #5 and 40 are plastome-encoded.
ND- not detected in autoradiograms.

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ISSN: 1471-2164

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