Figure 2

Differential expression of miRNAs and 21U-RNAs in each stage with and without the prg-1 mutation. (A) The proportion of changed miRNAs in six stages. “up” indicates that the expression was up-regulated more than twofold after prg-1 mutation; and “down” indicates that the expression was down-regulated more than twofold after prg-1 mutation. (B) All miRNAs were classified by the number of times (1 ~ 6) that a miRNA was decreased in different stages. The time distribution of the down-regulated miRNAs is shown for each stage. For example, the red bar shows the number of the down-regulated miRNAs that were down-regulated in five stages. Most of the down-regulated miRNAs were down-regulated in five or six stages after mutation of prg-1. Thus, the decrease in the miRNA levels was independent of the developmental stage. (C) The numbers of expressed 21U-RNAs in six stages of wild type and prg-1 mutant.