Expression of the unknown function ORFs in the hau CMS line and its maintainer line. (A) The expression of the hau CMS mitochondrial genome specific orf288, orf325 and orf170 in four different tissues. The CMS-associated gene orf288 is expressed in the flower, leaf, root and etiolated seedings of B. juncea and is carried by the hau CMS mitochondrial genome. However, orf325 did not appear in the root and orf170 only appeared in the leaf and etiolated seedings 7 days after germination. (B) orf109 and rps7 both appeared in the flower, leaf, and root. However, orf293, which is similar to orf288, only appeared in the leaf of hau CMS maintainer line.