Identification of target genes, which may be regulated by cell type specific enhancers. (A) UCSC genome browser screenshot for an example of active HSEL (red bar) and their putative target genes (red arrows) (B) UCSC genome browser screenshot for an example of active MSEL (red bar) and their putative target genes (red arrows) (C) Quantitative real-time RT-PCR analysis of putative target genes of active HSEL (i.e. MARVELD1, PI4K2A, AVIPI1) and active MSEL (i.e. KANK2, SPC24, LDLR). Two primer sets located in the exons of each gene were used, and the expression levels were presented relative to GAPDH expression. The error bars indicated the standard deviations from triplicate in HMEC (black) and MDAMB231 (grey). (D) Gene ontology process categories, which were differentially enriched in the putative target genes of active HSEL (black) and active MSEL (purple). x-axis indicated the -Log2 of p-value, calculated by performing chi-square between groups. The number of genes belonged to each GO category was shown on the right side of the bar.