Association of potential X-specific repeat in the exon duplication of papaya zinc finger protein. (A) Schematic demonstration of the duplication of X-specific repeat, X-R55, containing the 3rd exon of papaya zinc finger protein (accession ID: EX272522.1). (B) Phylogenetic tree of multiple X-R55 copies. The numbers at the root of each branch joining point are boot strap values. (C) Gel image of genomic PCR result for testing presence of the X-R55 (SF: SunUp female, SH: SunUp hermaphrodite, AU9F: AU9 Female, AU9M: AU9 male). (D) Gel image of RT-PCR result for testing the expression of papaya zinc finger protein in various tissues from SunUp and AU9 papaya (FF: female flower, FL: female leaf, HF: hermaphrodite flower, HL: hermaphrodite leaf, MF: male flower, ML: male leaf, SD: seeds, FT: 50% mature fruit). (E). Phylogenetic tree of papaya zinc finger protein (Cp zinc finger) with homologous proteins from other plant species with accession ID in NCBI.