Figure 4

Co-transcriptional cleavage of Trypanosoma spp ribozymes. The assayed RNA constructs are shown in (A). The striped region indicates the ribozyme region/s, and the grey regions indicate those that do not, a priori, contribute to ribozyme function (such as the large hairpin in TbSIDER2(162)Rz or the attenuator sequences in L1/NARTco(124)Rz). The results of co-transcriptional cleavage reactions with the unrelated pGEM-T easy 61 nucleotides and the natural upstream 20 nt-long sequence are shown in (B) and (C) respectively. The different cleavage products are presented using the same code as in Figure 3. The co-transcriptional cleavage quantification of the active ribozymes, performed in triplicate, is shown in (D).