The wide expansion of hepatitis delta virus-like ribozymes throughout trypanosomatid genomes is linked to the spreading of L1 Tc / ingi clade mobile elements

Table 1 Kinetic parameters of cleavage reactions

Ribozyme	R²	Two-phase		Plateau (%)				Co-transcriptional cleavage (%)
Ribozyme	R²	Hyperbolic		Plateau (%)				Co-transcriptional cleavage (%)
[MgCl₂] mM	10	1	0.1	10	1	0.1	0	6
Li/LdSIDER2A	0.9956	0.9932	0.9854	75.41	74.46	67.32	4.941	35.493 ± 0.402
Li/LdSIDER2A	0.9379	0.9099	0.8769	75.41	74.46	67.32	4.941	35.493 ± 0.402
LmSIDER2A	0.9184	0.9792	0.9975	73.58	61.54	82.37	-	38.622 ± 1.290
LmSIDER2A	0.8774	0.8956	0.9789	73.58	61.54	82.37	-	38.622 ± 1.290
LmexSIDER2A	0.4263	-	-	3.764	-	-	-	0.179 ± 0.008
LmexSIDER2A	0.3695	-	-	3.764	-	-	-	0.179 ± 0.008
TvSIDER1	0.9690	0.8169*	0.8335*	99.27^!	20.69	~14.62	~2.767	94.242 ± 1.076
TvSIDER1	0.8924	0.7130	0.3603	99.27^!	20.69	~14.62	~2.767	94.242 ± 1.076
L1/NARTco	0.9817	0.9576	0.9789	69.74^!	19.34	24.02	7.703	94.053 ± 0.181
L1/NARTco	0.8749	0.6885	0.7805	69.74^!	19.34	24.02	7.703	94.053 ± 0.181

Note: *means an ambiguous fitting, and ! means data probably over-estimated. In all cases, the ribozyme kinetics fitted the R² coefficient two-phase exponential decay model better than the hyperbolic model.

ISSN: 1471-2164