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Table 1 Kinetic parameters of cleavage reactions

From: The wide expansion of hepatitis delta virus-like ribozymes throughout trypanosomatid genomes is linked to the spreading of L1Tc/ingi clade mobile elements

Ribozyme R2 Two-phase Plateau (%) Co-transcriptional cleavage (%)
Hyperbolic
[MgCl2] mM 10 1 0.1 10 1 0.1 0 6
Li/LdSIDER2A 0.9956 0.9932 0.9854 75.41 74.46 67.32 4.941 35.493 ± 0.402
0.9379 0.9099 0.8769
LmSIDER2A 0.9184 0.9792 0.9975 73.58 61.54 82.37 - 38.622 ± 1.290
0.8774 0.8956 0.9789
LmexSIDER2A 0.4263 - - 3.764 - - - 0.179 ± 0.008
0.3695 - -
TvSIDER1 0.9690 0.8169* 0.8335* 99.27! 20.69 ~14.62 ~2.767 94.242 ± 1.076
0.8924 0.7130 0.3603
L1/NARTco 0.9817 0.9576 0.9789 69.74! 19.34 24.02 7.703 94.053 ± 0.181
0.8749 0.6885 0.7805
  1. Note: *means an ambiguous fitting, and ! means data probably over-estimated. In all cases, the ribozyme kinetics fitted the R2 coefficient two-phase exponential decay model better than the hyperbolic model.