Ribozyme
|
R2
|
Two-phase
|
Plateau (%)
|
Co-transcriptional cleavage (%)
|
---|
Hyperbolic
|
---|
[MgCl2] mM
|
10
|
1
|
0.1
|
10
|
1
|
0.1
|
0
|
6
|
Li/LdSIDER2A
|
0.9956
|
0.9932
|
0.9854
|
75.41
|
74.46
|
67.32
|
4.941
|
35.493 ± 0.402
|
0.9379
|
0.9099
|
0.8769
|
LmSIDER2A
|
0.9184
|
0.9792
|
0.9975
|
73.58
|
61.54
|
82.37
|
-
|
38.622 ± 1.290
|
0.8774
|
0.8956
|
0.9789
|
LmexSIDER2A
|
0.4263
|
-
|
-
|
3.764
|
-
|
-
|
-
|
0.179 ± 0.008
|
0.3695
|
-
|
-
|
TvSIDER1
|
0.9690
|
0.8169*
|
0.8335*
|
99.27!
|
20.69
|
~14.62
|
~2.767
|
94.242 ± 1.076
|
0.8924
|
0.7130
|
0.3603
|
L1/NARTco
|
0.9817
|
0.9576
|
0.9789
|
69.74!
|
19.34
|
24.02
|
7.703
|
94.053 ± 0.181
|
0.8749
|
0.6885
|
0.7805
|
- Note: *means an ambiguous fitting, and ! means data probably over-estimated. In all cases, the ribozyme kinetics fitted the R2 coefficient two-phase exponential decay model better than the hyperbolic model.