Figure 3

Higher reproducibility and better dynamics of differential expression detection using EXPRSS. (A-C) Pairwise scatter plots of gene counts from treatment replicates, expressed as tags per million in log10 scale. Correlations between four independent technical replicates of EXPRSS Tag-seq (A), made from same RNA sample; four independent biological replicates of EXPRSS Tag-seq (B) and NlaIII-DGE (C) are presented. Pearson correlation of log10 transformed tag counts per million plus 1 is depicted at left hand top corner of each comparison. Right hand bottom corner indicates replicate number depicted on X and Y-axis, respectively. (D-E) Example of differential expression using reads aligned (small black arrows) to WRKY22, a flg22 responsive gene are presented from control (D) and treatment (E).