Figure 4

Comparison of differential expression between EXPRSS, Nla III-DGE and Affymetrix ATH1 array. (A-B) Venn diagrams showing overlap of differential expression identified from the same RNA samples (EXPRSS and NlaIII-DGE) and similar RNA sample (ATH1 microarray). Overlap of (A) sense transcripts (numbers in black - genes spotted on ATH1 array and numbers in red- genes not spotted on ATH1 array) (B) antisense transcripts identified by EXPRSS and NlaIII-DGE. (C-D) Q-PCR confirmation of differential expression observed through EXPRSS. Three up-regulated genes (C) and five down-regulated genes (D) that are found differentially expressed with EXPRSS are verified with QPCR. Error bars indicate standard deviation from three biological replicates. FDR values are provided for EXPRSS log2 fold changes.