Experimental validation of the EPS network hierarchy. A: Total cell wall polysaccharides were extracted from respectively LGG wild-type, a ΔwelE::TcR gene deletion mutant (CMPG5351) and ΔwelI::TcR gene deletion mutant (CMPG10811). The total amount of EPS was measured. Error bars indicate standard deviations (of three repeats). One-way ANOVA statistical analysis rendered a p-value smaller than 0.05 for the variation of EPS across strains. B: Sugar monomer composition. The data are expressed as relative amounts, taking the total amount of detected monomeric sugars as 100%. Error bars indicate standard deviations (of three repeats). One-way ANOVA analyses (performed independently on each of the three datasets) rendered significant p-values (<0.05) for the variation of each sugar monomer across strains. C: Adhesion capacity. The adhesion capacity of wild type and mutants to Caco-2 cells is compared. Error bars indicate standard deviations (of three repeats). A One-way ANOVA analysis rendered a significant p-value (<0.05) for the variation of the adhesion capacity of the strains.