Figure 1

Determination of the transcriptional direction of pancRNAs and mRNAs. At the top of each panel, there is a schematic representation of promoter regions of Pacsin1 (A)(C) and Kcnmb4 (B)(D). Filled and open arrowheads represent the primer sets used for the strand-specific RT-PCR analysis for expression of pancRNAs and mRNAs, respectively. Detailed primer information is given in Additional file 1: Table S9. (A)(B) Strand-specific RT-PCR analysis for expression of mRNA and pancRNAs in the mouse cerebral cortex (Cx) and heart (Heart). (C)(D) RT-PCR analysis for expression of mRNA and pancRNAs in the mouse cerebral cortex. + and - mean the use and lack of reverse transcriptase in the reverse transcription (RT rxn).