Timing of life cycle gene expression. (A) Example spline and f’(x) curves for two genes, SporoSAG and GRA1. The maximum and minimum expression values were determined from the spline curve (arrows black curve). The maximum rate of synthesis and degradation were determined from the f’(x) of the spline curve (arrows red curve). (B) Number of genes with maximum or minimum expression across the life cycle calculated from spline curves for all 5,969 life cycle regulated genes. (C) Maximum synthesis and degradation rates across the life cycle calculated from f’(x) of spline curves for all 5,969 life cycle regulated genes. (D) For each of the 5,969 regulated genes the expression value (B) was subtracted from the respective synthesis value (C) to determine time to maximum or minimum expression. Histograms displayed at half stage intervals for B-D.