Motif discovery in promoter regions of life cycle regulated genes. (A) Life cycle regulated genes (5,969) were ordered by point of maximum expression and the FIRE software package was used to find motifs over- and under-represented in sequence 2,000 bp 5’ of the CDS start. Motifs over-represented at a particular point in the life cycle (yellow), those under-represented (blue). (B) Life cycle regulated genes were ordered by point of minimum expression and run in FIRE as in (A). The TGCATGC motif found in both (A) and (B) was also a major motif found in the Toxoplasma cell cycle expression data set  where, for example, it was over-represented at G1 for genes with maximum expression at that phase (inset). No significant motifs were found across 100 FIRE runs when data in (A) or (B) were randomly ordered.