EMS generated mutants. (A) The incidence of various SNVs in protein coding regions versus those outside annotated open reading frames. Syn.: synonymous; non-syn: non-synonymous. The number between brackets is the percentage of mutations in each category. (B) The incidence of SNVs across the EMS mutants screened for resistance to pharmacologically induced egress using DTT (E2D2 and E4D5) or invasion enhancing compound 2 (E3E2) [42, 43]. These three mutants were generated using an EMS dosage inducing 70% killing. (C) The incidence of SNVs across the EMS mutants screened for resistance against 20 μM FUDR . EMS7.5 and EMS10 were generated using 7.5 and 10 mM EMS, inducing 80% and 90% killing, respectively.