Figure 7

Genome wide distribution of mutations. (A) Circos plot of all mutants and gene density. The Toxoplasma GT1 reference genome is composed of the 14 chromosomes displayed in the outermost circle. The SNVs shared between all 3 RH parent lines and the GT1 reference are represented by green dots. Strain and mutant specific variations are represented by black dots and are grouped as either parent lines (yellow) or by mutant genealogy and mutagen as shown in Figure 1. Gene density was mapped using a sliding window approach with a window size of 100 kb. (B-D) QQ-plot of SNV distances vs. exponential distribution. To test the assumption that mutations are distributed randomly, the inter-SNV distances (taken from Additional file 2: Table S2) are plotted against the exponential distribution, with λ = 1/mean, using a Quantile-Quantile plot; B = distances between ENU induced SNVs (N = 368, mean = 159582), C = distances between EMS induced SNVs (N = 144, mean = 359992.3), D = distances between shared SNVs between the parent strains (N = 968, mean = 61631.86). The solid red line in each plot represents the null assumption y = x.