Figure 3

Relative abundances of Pfams within the metagenome and metasecretome-enriched sequence datasets. Relative abundances of IMG/M annotated COG-based functional categories of protein family (Pfam) conserved domains within the metagenome (purple bars) and metasecretome-enriched (green bars) sequence datasets. Abbreviations for the functional categories, grouped by general functional role: Information storage and processing (blue font): J – Translation, ribosomal structure and biogenesis, A – RNA processing and modification, K – Transcription, L – Replication, recombination and repair, B – Chromatin structure and dynamics; Cellular processes and signalling (red font): D – Cell cycle control, cell division, chromosome partitioning, Y – Nuclear structure, V – Defence mechanisms, T – Signal transduction mechanisms, M – Cell wall/membrane/envelope biogenesis, N – Cell motility, Z – Cytoskeleton, W – Extracellular structures, U – Intracellular trafficking, secretion and vesicular transport, O – Posttranslational modification, protein turnover, chaperones; Metabolism (green font): C – Energy production and conversion, G – Carbohydrate transport and metabolism, E – Amino acid transport and metabolism, F – Nucleotide transport and metabolism, H – Coenzyme transport and metabolism, I – Lipid transport and metabolism, P – Inorganic ion transport and metabolism, Q – Secondary metabolites biosynthesis, transport and catabolism; Poorly characterized (grey font): R – General function prediction only, S – Function unknown. Significant difference between metasecretome and metagenome datasets within given functional category is represented by asterisks (* P ≤ 0.001).