Transcriptomic changes during regeneration of the central nervous system in an echinoderm

BMC Genomics

Table 3 Validation of assembled contigs by re-sequencing using Sanger technology

ContigID	Best blastx	ORF complete?	Frameshifts	Contig length, bp	Checked by Sanger	Insertions	Deletions	Substitutions	% Discrepancy
	match		in ORF		sequencing, bp
Contig3756	CENPS	yes	no	665	564	8	0	1	1.60%
Contig7869	Myc	yes	no	3,268	2,922	0	0	0	0.00%
Contig54848	HuD/Elav	yes	1	4,404	3,431	9	2	3	0.41%
Contig19303	Lgr5	both 5’ and 3’ ends are missing	no	1,510	1,510	0	0	0	0.00%
Contig40997	NeuroD	both 5’ and 3’ ends are missing	no	745	470	0	1	2	0.64%
Contig1064	NF1A	yes	no	5,279	3,825	3	9	1	0.34%
Contig32193	NvProtien1	5’ end is missing	no	2,411	1,986	0	1	0	0.05%
Contig51632	Oct	both 5’ and 3’ ends are missing	no	1,947	1947	0	0	0	0.00%
Contig46302	Piwi	yes	no	3,430	2,929	0	0	3	0.10%
Contig67140	Sox	yes	no	3,096	2,889	2	0	0	0.07%
Contig20197	unknown	NA	NA	1,339	1,252	2	0	0	0.16%
Contig68289	Wnt3	yes	no	2,561	990	2	0	2	0.40%
Contig50192	DCLK	yes	no	3,152	2,474	0	0	3	0.12%
Contig8186	Lhx	yes	no	5,430	3,041	0	0	5	0.16%
Contig3071	FoxJ1	yes	no	2,523	2,404	2	3	0	0.21%
Contig10179	Klf	yes	no	2,336	2,074	1	0	0	0.05%
			TOTAL:	44,096	34,708	29	16	20	0.19%

ISSN: 1471-2164