Figure 4

Heat map representation of the transcriptional response of the S. mutans pyrimidine metabolic pathway upon carolacton treatment. Genes from the pyrimidine metabolism pathway were among the first to be modulated upon carolacton treatment. The log2-fold expression change of pathway genes at 5 min post treatment were used for the heat-map representation. Green indicates upregulation and red downregulation. The scale is indicative of the corresponding changes in normalized gene expression. Pathway genes encoding enzymes catalyzing reactions leading up to UMP were strongly upregulated while most of the other pathway genes exhibited relatively weak modulation. Enzymes marked in black bold rectangles indicate the corresponding strongly upregulated transcripts of the pathway. White cells correspond to pathway enzymes not found in the genome of S. mutans UA159. If a particular enzyme corresponds to multiple transcripts (as a result of multiple protein subunits constituting an enzyme), then the transcript with the highest amplitude of log2-fold change was used. Graph generated using the Mayday visualization tool version 2.12.