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Figure 6 | BMC Genomics

Figure 6

From: Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis

Figure 6

Functional characterization and verification of the modification genes in the vcm cluster. The 64-kb vcm cluster is illustrated in detail. AORI_1490 (vmt), AORI_1486 (gtfD), AORI_1487 (gtfE), and AORI_1485 (vhal) were replaced in-frame by selection markers, and AORI_1490 was overexpressed in vitro using demethylvancomycin/aglucovancomycin as the substrate. The vancomycin standards (A) and the corresponding variants obtained by isolation from mutant strains or the in vitro treatments were detected by HPLC-MS: (B) dechlorovancomycin, (C) desvancosamine vancomycin, (D) aglucovancomycin, (E) dimethylaglucovancomycin, (F) demethylvancomycin, and (G) dimethylvancomycin. The structural formulae of the variants are shown in the table of Figure 1. A mass of 20 μg of each compound was used to assay its activity against MRSA, and the picture is representative of three independent experiments.

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