Flowchart of the de novo transcript assembly process. The Western black widow transcriptome was assembled in three major steps. First, high-quality 75 or 100 base paired-end cDNA sequence reads were generated for each of three tissues (dark blue boxes). Second, transcripts were de novo assembled for each tissue separately (light blue boxes). Finally, the high quality non-redundant transcriptome was generated (yellow boxes). Relevant programs are shown parenthetically in the boxes. The number of sequence reads, Trinity de novo assembled sequences, and final assembled transcripts generated in each step are shown in bold below the arrows.