Maximum likelihood trees of cluster containing black widow aggregrate gland silk factor 2 (AgSF2). (A) Tree of unique assembled transcripts (UATs) that includes published L. hesperus (Lh) AgSF2 (orange). (B) Tree of translated UATs that clustered with UATs in (A) based on amino acid composition, rather than true homology. A third group of three UATs that was also in this cluster is not shown. Trinity derived isoforms of a single component are shown in dark blue. The trees are midpoint rooted. All bootstrap values greater than 50 are shown above branches. Expression rank and FPKM (fragments per thousand base pairs per million mapped fragments) are shown on the right. Rank is based on FPKM in the silk glands out of all UATs expressed in silk glands.