Multi-tissue transcriptomics of the black widow spider reveals expansions, co-options, and functional processes of the silk gland gene toolkit

Table 1 Silk gland-specific unique assembled transcripts (UATs) predicted to encode transcription factors

Western black widow SST^a	PFAM motif ID^b	Fruit fly homolog^c	Fruit fly tissue^d	Fruit fly interaction partners^e	UATs homologous to interaction partners^f
silk_Contig619	NA (homeobox)	FBgn0001235	L3 CNS	4	13 (0)
Contig373	Fork_head	FBgn0000659	6-8 hr Embryo	0	0
Contig303	T-Box	FBgn0261963	16 hr Embryo	0	0
silk_comp12525_c0_seq1	Homeobox	FBgn0000014	16 hr Embryo	2	6 (0)
Contig41	LIM	FBgn0026411	16 hr Embryo	0	0
silk_comp6942_c0_seq1	Homeobox	FBgn0003944	16 hr Embryo	20	64 (0)
Contig5112	TAFH	No match	NA	NA	NA
silk_comp16984_c0_seq1	Homeobox_KN	FBgn0001235	Larval stage 3 CNS cells	4	13 (0)
silk_comp16140_c0_seq1	Homeobox	FBgn0000015	S3 Cells	0	0
silk_comp15765_c0_seq1	Homeobox	FBgn0000015	S3 Cells	0	0

^aSST = silk-specific transcript. See Methods for identification of SSTs.
^bPFAM ID was assigned using highest match to a domain with HMMer 3.0.
^cFruit fly, Drosophila melanogaster, proteins were from the reference sequences (RefSeq) available in NCBI as of July 2012.
^dFruit fly tissues with the highest FPKM support were selected from FlyBase FPKM report obtained April 2013.
^eInteraction partners are fruit fly genes noted in the FlyBase Physical Interaction Database obtained in April 2013 as interacting with the fruit fly homolog of black widow SSTs.
^fHomologous black widow UATs to fruit fly interaction partners were chosen by best BLASTX match (SSTs in parentheses).

ISSN: 1471-2164